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Composition of Promega Restriction Enzyme Reaction Buffers 1X
For ordering information on the products discussed here, please visit our Restriction Enzymes product listing.
Promega Restriction Enzyme Reaction Buffers are provided as 10X stock solutions. This reference table provides compositions for Restriction Enzyme Reaction Buffers, listed as 1X concentrations.
| Buffer | pH (at 37°C) |
Tris-HCl (mM) |
MgCl2 (mM) |
NaCl (mM) |
KCl (mM) |
DTT (mM) |
|---|---|---|---|---|---|---|
| A | 7.5 | 6 | 6 | 6 | – | 1 |
| B | 7.5 | 6 | 6 | 50 | – | 1 |
| C | 7.9 | 10 | 10 | 50 | – | 1 |
| D | 7.9 | 6 | 6 | 150 | – | 1 |
| E | 7.5 | 6 | 6 | 100 | – | 1 |
| F | 8.5 | 10 | 10 | 100 | – | 1 |
| G | 8.2 | 50 | 5 | – | – | – |
| H | 7.5 | 90 | 10 | 50 | – | – |
| J | 7.5 | 10 | 7 | – | 50 | 1 |
| K | 7.4 | 10 | 10 | – | 150 | – |
| L | 9.0 | 10 | 3 | 100 | – | – |
MULTI-CORE™ Buffer (1X) = 25mM Tris-Acetate, pH 7.5 (at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT.
Notes:
- For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.
- For each 10°C rise in temperature between 25°C and 37°C, the pH of Tris buffers decreases 0.25 pH units.