pCI-neo Mammalian Expression Vector

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The vector is maintained as an episome in cells expressing the SV40 large T antigen, leading to even higher levels of expression.

This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Multiple cloning sites exist for easy insertion of cDNA.

References

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4. Carswell, S. and Alwine, J.C. (1989) Mol. Cell. Biol. 9, 4248–58.