In vitro Transkription
RNA-Produktionssysteme, Consensus-Oligos, Vektoren und Ribonukleotide für in vitro Expressionsstudien.
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Produktübersicht zu In vitro Transkription
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Compare Riboprobe®, RiboMAX™ and RiboMAX™ Express System features and RNA yields.
| RiboMAX™ AOF RNA Production System | T7 RiboMAX™ Express Large Scale RNA Production System | RiboMAX™ Large Scale RNA Production Systems | Riboprobe® Systems |
T7 RiboMAX™ Express RNAi System | |
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| Time | |||||
| RNA Yield (per 1ml reaction) | ≥2mg dsRNA |
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| AOF/cGMP Reagents | |
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| Compatible with Modified Nucleotides | |
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| T7 Promoter | |
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| SP6 Promoter | |
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| T3 Promoter | |
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| Radiolabeling | |
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NT = not tested.
An Introduction to RNA Synthesis Methods
In vitro RNA synthesis methods use bacteriophage DNA-dependent RNA polymerase such as T7, T3 or SP6 RNA polymerase to synthesize RNA from a DNA template. The template DNA for in vitro transcription reactions includes an RNA polymerase promoter upstream of the sequence of interest. The corresponding RNA polymerase is then used to produce synthetic RNA transcripts for use as hybridization probes, as templates for in vitro translation applications, or in structural studies (X-ray crystallography and NMR). Synthesized RNA transcripts are also used for studying cellular RNA functionality in processes such as splicing, RNA processing, intracellular transport, viral infectivity and translation.
