DPPIV-Glo™ Protease Assay

The DPPIV-Glo™ Assay measures dipeptidyl peptidase IV (DPPIV) activity can be used to determine DPPIV enzyme activity or perform kinetic studies of DPPIV inhibitors. DPPIV is a serine protease that cleaves N-terminal dipeptides from polypeptides with l-proline or l-alanine at the penultimate position. The assay provides a proluminescent DPPIV substrate, Gly-Pro-aminoluciferin, in a buffer system optimized for DPPIV and luciferase activities. The addition of a single DPPIV-Glo™ Reagent in an "add-mix-measure" format results in DPPIV cleavage of the substrate and generation of a "glow-type" luminescent signal produced by the luciferase reaction. In this homogeneous, coupled-enzyme format, the signal is proportional to the amount of DPPIV activity present. The assay is designed for use with purified enzyme preparations.

• Simplified method is highly amenable to automation.
• More sensitive than fluorescent-based DPPIV assays; the luminescent assay avoids inherent fluorescent background signals and thus provides excellent signal-to-background readings. The assay is linear over more than 3 logs of DPPIV concentration and can detect less than 1pg/ml enzyme.
• Maximum signal (and maximum sensitivity) of the assay is reached in as little as 30 minutes after reagent addition and, unlike fluorescent assays, is not dependent on accumulation of cleaved product.
• Amenable to batch processing with a stable signal that allows plates to be read over an extended period of time.

Number of Assays Per Plate