Lys-C, Mass Spec Grade

Active Under Strong Denaturing Conditions

Endoproteinase Lys-C, Mass Spec Grade, is a highly-purified serine protease that hydrolyzes specifically at the carboxyl side of lysines. Lys-C retains proteolytic activity under strong protein denaturing conditions such as 8M urea, which can be used to improve digestion of proteolytically resistant proteins. Lys-C, Mass Spec Grade, has optimal activity in the range of pH 7.0—9.0. This protease can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching.

Digestion Under Strong Denaturing Conditions

Native Lys-C Interchangeable with Recombinant Lys-C

Comparison of recombinant and native Lys-C protein digestions. — **Comparison of lysine-specific cleavages.** Mass Spec-Compatible Yeast Protein Extract, Intact (Cat.# V7341) was digested with either rLys-C (*P. aeruginosa*; Cat.# V1671) or native Lys-C (*Lysobacter sp*.; Cat.# VA1170) at a ratio of 1:100.

Protokolle

Complete Protocol

Lys-C, Mass Spec GradePDF (104 KB) – English

Lieferumfang

Produkt	Katalognummer	Größe
Lys-C Mass Spec Grade	VA117A	1 × 20μg

SDS

Search for SDS

Analysezertifikat

Search by lot number

Nutzungseinschränkung

For Research Use Only. Not for Use in Diagnostic Procedures.

Lagerhinweise

Wird oft zusammen gekauft

IdeS and IdeZ Proteases

IgG-degrading enzymes that cleave at a single site below the hinge region.

V7511, V7515, V8341, V8345

Magnetic Proteomics Sample Prep (MPSP) Kit

Magnetic beads for streamlined, SP3-based proteomics sample preparation.

CS3325A04

Lys-C, Mass Spec Grade

Part Numbers: VA1170

Protease for Challenging Protein Digestions

Active Under Strong Denaturing Conditions

Digestion Under Strong Denaturing Conditions

Native Lys-C Interchangeable with Recombinant Lys-C

Protokolle

Complete Protocol

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

rChymoSelect™ MS Grade Protease Kit

Arg-C Ultra, Mass Spec Grade

rAsp-N, Mass Spec Grade

Glu-C, Sequencing Grade

Chymotrypsin, Sequencing Grade

Lys-C, Mass Spec Grade

Part Numbers: VA1170

Protease for Challenging Protein Digestions

Active Under Strong Denaturing Conditions

Digestion Under Strong Denaturing Conditions

Native Lys-C Interchangeable with Recombinant Lys-C

Protokolle

Complete Protocol

Eigenschaften

Lieferumfang

SDS

Analysezertifikat

Nutzungseinschränkung

Lagerhinweise

Ähnliche Produkte

rChymoSelect™ MS Grade Protease Kit

Arg-C Ultra, Mass Spec Grade

rAsp-N, Mass Spec Grade

Glu-C, Sequencing Grade

Chymotrypsin, Sequencing Grade

Wird oft zusammen gekauft

IdeS and IdeZ Proteases

Magnetic Proteomics Sample Prep (MPSP) Kit

Americas

Pacific Asia

Europe