Glu-C, Sequencing Grade
Cleaves at the C-Terminus of Either Aspartic or Glutamic Acid Residues
- Optimal activity at pH 4–9
- Ammonium and phosphate buffers can affect cleavage; see details in product information
- Suitable for in-solution digestion; not recommended for in-gel digestion
Catalog Number:
Size
Catalog Number: V1651
This sequencing grade serine protease (S. aureus V8) specifically cleaves at the C-terminus of either aspartic or glutamic acid residues. In ammonium bicarbonate and ammonium acetate the enzyme specificity is higher at the glutamic residues. In phosphate buffers cleavage occurs at the aspartic and glutamic residues. Glu-C activity is optimal in the pH range of 4.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in solution but not recommended for in-gel digestions. (S. aureus V8)
Applications- Peptide mapping
- Protein identification
Protocols
Complete Protocol
Specifications
Catalog Number:
Lieferumfang
| Produkt | Katalognummer | Größe |
|---|---|---|
|
Glu-C, Sequencing Grade |
V165A | 5 × 10μg |
SDS
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Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
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