Asp-N, Sequencing Grade
Hydrolyzes Peptide Bonds on the N-Terminal Side of Aspartic and Glutamic Acid Residues (Asp and Glu)
- Optimal activity in pH range of 4–9
- Sequencing grade enzyme for use alone or in combination with other proteases
- Use for in-solution or in-gel digestion reactions
Catalog Number:
Size
Catalog Number: V1621
This endoproteinase hydrolyzes peptide bonds on the N-terminal side of aspartic and to a lesser extent glutamic acid residues (Asp and Glu). Asp-N activity is optimal in the pH range of 4.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in solution or in gel.
Applications
- Peptide mapping
- Protein identification
Protocols
Complete Protocol
Specifications
Catalog Number:
Lieferumfang
| Produkt | Katalognummer | Größe |
|---|---|---|
|
Asp-N, Sequencing Grade |
V162A | 1 × 2μg |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
For Research Use Only. Not for Use in Diagnostic Procedures.Lagerhinweise
Resources
No related resources available
Related Products
Ähnliche Produkte
Endoglycosidase H
Characterize protein glycosylation in applications such as monitoring protein trafficking and determining glycan location and structure by mass spectrometry.
V4871, V4875
PNGase F
Catalyzes cleavage of N-linked oligosaccharides between the innermost GlcNAc and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins.
V4831
rAsp-N, Mass Spec Grade
Cleavage site: N-terminal of Asp. Optimal pH: 6–9.
VA1160
Trypsin Gold, Mass Spectrometry Grade
Maximum digest specificity with extreme resistance to autolytic digestion
V5280
Trypsin/Lys-C Mix, Mass Spec Grade
A mixture of Trypsin Gold, Mass Spec Grade, and rLys-C, Mass Spec Grade, designed to improve digestion of proteins or protein mixtures in solution.
V5071, V5072, V5073
ProteaseMAX™ Surfactant, Trypsin Enhancer
For improved in-gel digestion and protein solubilization.
V2071, V2072
Wird oft zusammen gekauft
Rapid-Digestion Trypsin and Trypsin/Lys-C Kits
Streamlined protocol for fast, efficient protein digestion.
VA1060, VA1061
Glu-C, Sequencing Grade
Cleaves at the C-terminus of either aspartic or glutamic acid residues and has optimal activity at pH of 4.0–9.0.
V1651
rLys-C, Mass Spec Grade
Cleaves at the carboxyl side of lysines. Retains proteolytic activity under denaturing conditions such as 8M urea, used with proteolytically resistant proteins.
V1671
Sequencing Grade Modified Trypsin
Trypsin manufactured for maximum specificity.
V5111, V5117, V5113
