VersaPlex® 27PY System
6-Color Co-Amplification and Detection of 27 Loci Including D6S1043
- Improved results from challenging samples helps save time and reduce costs by minimizing the need to repeat assays
- Designed for forensics casework and paternity determination
- Rapid cycling capabilities reduce processing time for all samples
Catalog Number:
Size
Catalog Number: DC7020
Overview
Short tandem repeat (STR) loci are well distributed throughout the human genome and can be detected using the polymerase chain reaction (PCR). Alleles of STR loci are differentiated by the number of copies of the repeat sequence contained within the amplified region. They can be distinguished from one another using fluorescence detection following electrophoretic separation.
The VersaPlex® 27PY System is a 6-color, 27-loci multiplex STR amplification system capable of rapid cycling. In addition to the D6S1043 locus, the kit includes: CSF1PO, FGA, TH01, TPOX, vWA, D1S1656, D2S441, D2S1338, D3S1358, D5S818, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11, D22S1045, Penta D, Penta E, DYS391, DYS570, DYS576 and Amelogenin.
Promega forensic products are manufactured in alignment with the ISO 18385 standard.This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.
Superior Discrimination
The VersaPlex® loci offer Probability of Identity (PI) values of 4.4 × 10–30 for four combined American populations and 6.2 × 10–27 for the Asian-American population.† Eleven loci are under 250bp, making the kit useful for degraded samples.
†Butler, J.M. et al. (2012) Variability of new STR loci and kits in US population groups. Profiles in DNA.
Configuration of the VersaPlex® 27PY System. The VersaPlex® 27PY System allows co-amplification and detection of 27 loci, including: D3S1358, D1S11656, D2S441, D10S1248, D13S317, D16S539, D18S51, D2S1338, CSF1P0, TH01, vWA, D21S11, D7S820, D5S818, TP0X, D8S1179, D12S391, D19S433, D6S1043, D22S1045, FGA, DYS391, as well as DYS576, DYS570, Penta D, Penta E and Amelogenin.
Improved Interpretation of Complex Mixtures
|
Y-STR
|
Number of Allelesa
|
Gene Diversitya
|
Mutation Rateb
|
|
|---|---|---|---|---|
|
DYS391
|
10
|
0.521
|
5/1759
|
3.23 × 10–3
|
|
DYS576
|
21
|
0.789
|
24/1727
|
1.43 × 10–2
|
|
DYS570
|
28
|
0.803
|
17/1429
|
1.24 × 10–2
|
|
a. Purps, J. et al. (2014) A global analysis of Y-chromosomal haplotype diversity for 23 STR loci. Forensic Sci. Int. Genet. 12, 12.
b. Ballantyne, K. et al. (2010) Mutability of Y-chromosomal microsatellites: rates, characteristics, molecular bases, and forensic implications. Am. J. Hum. Genet. 87, 341. |
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Electropherogram of a three-person mixture. A complex three-person DNA mixture with 45pg each of two different minor male contributors and one major female contributor (1ng total) was amplified for 29 cycles. Amplified samples were separated on a 3500xL Genetic Analyzer using a 1.2kV 24-second injection. Data were analyzed with GeneMapper® ID-X software.
Wide Range of Sample Inputs
While optimized at 1ng of input DNA, the VersaPlex® 27PY System can handle a wide range of input amounts. The figures below show that the VersaPlex® 27PY System generated a complete, balanced profile at twice the recommended input DNA concentration compared to a competitive kit that exhibited saturation at twice its recommended input.
VersaPlex® 27PY System with 2ng Input
VeriFiler® Plus Kit with 1ng Input
VersaPlex® 27PY System performance compared to a competitive kit. Amplified samples were separated on a 3500xL Genetic Analyzer using a 1.2kV 24-second injection. Data were analyzed with GeneMapper® ID-X software.
High Sensitivity for More Complete Profiles
The VersaPlex® 27PY System is highly sensitive. Full profiles can be generated at 62.5pg and more than 92% of alleles can be called with as little as 30pg of DNA.
Percentage of alleles called using three different multiplexes. Following recommend protocols for each kit, varying input amounts of DNA were amplified. Amplified samples were then separated on a 3500xL Genetic Analyzer with a 1.2kV 24-second injection. Data were analyzed using a 100RFU threshold for all three chemistries using GeneMapper® ID-X software.
Protocols
Complete Protocol
Kurzprotokoll
Specifications
Catalog Number:
Lieferumfang
| Produkt | Katalognummer | Größe | Konzentration | Available Separately |
|---|---|---|---|---|
|
2800M Control DNA |
DD710A | 1 × 25μl | 10ng/μl | View Product |
WEN Internal Lane Standard 500 |
DG500A | 2 × 200μl | View Product | |
VersaPlex® 27PY Allelic Ladder Mix |
DG826B | 1 × 100μl | ||
|
Water, Amplification Grade |
DW099A | 5 × 1,250μl | View Product | |
VersaPlex® 27PY 5X Master Mix |
DP307A | 1 × 1ml | ||
VersaPlex® 27PY 5X Primer Pair Mix |
DK828B | 1 × 1ml |
SDS
Search for SDSAnalysezertifikat
Nutzungseinschränkung
Not For Medical Diagnostic Use.Lagerhinweise
U.S. Pat. No. 9,139,868, European Pat. No. 2972229, Japanese Pat. No. 6367307 and other patents pending.
TMR, CXR, TOM and WEN dyes are proprietary.
Resources
Software und Firmware
Zusätzliches Informationsmaterial
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