Cut Site:
T▼CG A
A GC▲T
Note: Sensitive to overlapping dam methylation.
Incubation Conditions: Buffer E. 65°C. (TaqI exhibits <10% activity at 37°C.)
Source: Thermus aquaticus YTI.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.