RNase ONE™ Ribonuclease

RNase ONE™ Ribonuclease is a 27kDa periplasmic enzyme from E. coli that catalyzes the degradation of RNA to cyclic nucleotide monophosphate (NMP) intermediates. Slower hydrolysis further catalyzes the degradation of these intermediates to 3´-NMPs. RNase ONE™ Ribonuclease may be used to remove RNA from DNA preparations, for mismatch detection, RNase protection assays and mapping, or quantitation of RNA by selective cleavage of single-stranded regions. The enzyme is provided with 10X Reaction Buffer: 100mM Tris-HCl (pH 7.5 at 25°C), 50mM EDTA, 2M sodium acetate.

1. Shen, V. and Schlessinger, D. (1982) The Enzymes, Vol. XV, 501.
2. Meador, J., 3rd et al. (1990) Eur. J. Biochem. 187, 549–53.
3. Meador, J., 3rd and Kennell, D. (1990) Gene 95, 1–7.

Storage Buffer: 10mM Tris-HCl (pH 8.0), 200mM NaCl, 50% (v/v) glycerol.

Source: Recombinant E. coli strain.

QC Tests: Activity, SDS-PAGE/purity, endonuclease, DNase.

Unit Definition: One unit is defined as the amount of enzyme required tocompletely degrade RNA at the rate of 100ng/second at 37°C in 10mM Tris-HCl (pH 7.5 at 25°C), 5mM EDTA, 200mM sodium acetate, 0.2μg/μl RNA, 0.05% NP-40 and 2μg/μl BSA.