LAG-3/MHCII Blockade Bioassay, Propagation Model Technical Manual
Instructions for Use of Product(s)
JA1112
Literature # TM571
The LAG-3/MHCII Blockade Bioassay is a bioluminescent cell-based assay that measures potency and stability of antibodies and other biologics designed to block the interaction of LAG-3 with its ligand, MHCII. LAG-3, also known as CD223, is an immune checkpoint receptor expressed on activated CD4+ and CD8+ T cells and natural killer (NK) cells. LAG-3 plays a critical role in regulating immune responses to tumor antigens and autoantigens. Engagement of LAG-3 by its best characterized ligand, major histocompatibility complex II (MHCII), inhibits TCR signaling, cytokine production and proliferation of activated T cells. Therapeutic antibodies designed to block the LAG-3/MHCII interaction show promising results in clinical trials for the treatment of a variety of cancers.
The LAG-3/MHCII Blockade Bioassay, Propagation Model (Cat.# JA1112) is a bioluminescent cell-based assay that measures the potency and stability of antibodies and biologics targeting LAG-3/MHCII and overcomes the limitations of existing assays. The LAG-3 Effector Cells and MHCII APC Cells are provided in a Cell Propagation Model (CPM) format, which includes cryopreserved cells that can be thawed, propagated and banked for long-term use (also offered in a thaw-and-use format; Cat.# JA1111, JA1115).
Summary of Changes
The following changes were made to the 7/24 revision of this document:
1. Text defining ICH was corrected in the Description.
2. Miscellaneous text edits were made.
3. Related Products was updated.
4. One patent statement and third party trademarks were updated.
5. The cover image and font were updated.
Revised 7/24.