GloMax® Discover Microplate Reader
Multimode Microplate Reader with Advanced Detection Capabilities
- Luminescence, fluorescence, UV-visible absorbance, BRET and FRET detection
- 6- to 384-well microplate reader
- Optimized with Promega cell viability and genetic reporter assays
Catalog Number:
Size
Catalog Number: GM3000
Easy-to-Use Plate ReaderÂ
GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance.
- High-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities
- Can be used as a standalone plate reader or integrated into high-throughput automated workflows
- Integrated data analysis software makes results easy to interpret
Preloaded Protocols for Faster, Easier Data AnalysisÂ
GloMax® Instruments Come with Dozens of Preloaded Protocols
GloMax® Application Notes
No need to reinvent the wheel—we’ve already used GloMax® instruments for everything from cell viability, toxicity and metabolism, kinase and cell signaling, reporter gene assays, nucleic acid quantitation for NGS sequencing, PROTACs and protein degradation, bioassays, BRET, ELISAs and even water quality testing. Search our Application Notes collection for examples using GloMax® instruments with a wide range of bioluminescence- and fluorescence-based assays.
Streamline Your Workflow with Automation
GloMax® Discover integrates with many commercially available automated systems, including liquid handers, plate stackers and robotic arms to enable seamless workflows for high-throughput assay quantitation and screening.
Our built-in and compliant SiLA2 communication driver makes integration easy.
Microplate Reader Compliance for Your GxP Workflow
Not sure if GloMax® Discover is the right plate reader for your lab?
Find the instrument that's right for your needs. Compare Plate Readers
Service and Support
One Call Supports It All
Promega supplies both the reagents and the instrument, so one call to Promega answers any questions you may have about assay chemistries or instrument performance.
Ensure Minimal Instrument Downtime
- Field Support and Loaner Programs
- Service Packages
- IQ and OQ Packages
Warranty and Service Agreements
- Backed by a one-year warranty.
- Additional warranty and service agreements are available.
- For more information contact Promega Technical Services
Design Features
| Detection Modes | Luminescence, Fluorescence Intensity, UV-Visible Absorbance, Filtered Luminescence, BRET and FRET |
| Sample Format | 6-, 12-, 24-, 48-, 96- and 384-well plates |
| Tablet PC | Windows® 8.1 or 10 Tablet PC with USB connection to instrument |
| Read Speed | 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes |
| Automation Compatibility | Hardware and software accessible to third-party hardware/software control, including LIMS. |
| Dimensions |
18.5in (46.9cm) width 19.5in (46.5cm) depth 10in (25.4cm) height without Tablet and PC mounted 16in (35.5cm) height with Tablet and PC mounted |
| Weight | Approximately 60lb (27kg) |
| Power Requirements | 120VA, 50/60HZ |
| Warranty | 1-year parts and labor warranty included |
| Regulatory Compliance | For Research Use Only. Not for use in Diagnostic Procedures |
| Detector | Top-reading, head-on photon counting photomultiplier tube (PMT) |
| Wavelength Range | 350–700nm |
| Detection Limit | 3 × 10–21 moles of luciferase |
| Linear Dynamic Range | 9 logs |
| Cross-talk | Less than 3 × 10–5 (white, 96-well plate, Corning 3912) |
| Filtered Luminescence | Built-in filter paddle |
| Detector | PIN-photodiode |
| Light Source | Wavelength-matched LED |
| Read Position | Top reading |
| Wavelengths selection | Filter module with 5 standard excitation and emission filters. Empty filter positions are available for custom excitation and emission wavelengths. |
| Wavelengths included | UV (Ex: 365nm, Em: 415–445nm) Blue (Ex: 475nm, Em: 500–550nm) Green (Ex: 520nm, Em: 580–640nm) Red (Ex: 627nm, Em: 660–720nm) AFC (Ex: 405nm, Em: 495–505nm) |
| Detection Limit | 2fmol fluorescence dye/200µl |
| Linear Dynamic Range | >6 logs (assay-dependent) |
| Detector | Top-reading, head-on photon counting photomultiplier tube (PMT) |
| Light Source | Xenon flash lamp |
| Spectra Range | 200–600nm |
| Filter Wheel | 9 absorbance filters included |
| Wavelengths included | 230, 260, 280, 320, 405, 450, 490, 560 and 600nm 10nm band pass |
| Detection Limit | 0.1 O.D. |
| Dynamic Range | 0–4.0 O.D. |
| Number of injectors (optional) | Two injectors |
| Dispense Volume Range | 5–200µl in 1µl increments |
| Plate Compatibility | 6-, 12-, 24-, 48- and 96-well plates |
| Injection Speed | 20–500µl per second |
| Waste Collection Tray Volume | Approximately 50ml |
| Void Volume | 600µl |
| Temperature Range | Ambient to 45°C |
| Shaking Pattern | Linear or orbital patterns |
| Shaking Intensity | 100–500 cycles/minute |
| Operating System | Windows® 8.1 or 10 Professional version, 64-bit, with USB connection to instrument |
| RAM | 4GB |
| Disk Space | 64GB |
| Other Requirements | Microsoft.NET 4.6.1 Framework or newer (full version) |
Available Separately
| Product | Catalog # |
|---|---|
| Luminometer Light Plate | E6531 |
| Bar Code Reader | AS3200 |
| USB Ethernet Adapter | AS8403 |
| GloMax® Discover Empty Luminescence Filter Paddle | GM3011 |
| GloMax® Discover Empty Fluorescence Filter Paddle | GM3012 |
| GloMax® Discover Luminescence Filter Paddle | GM1090 |
| GloMax® Discover Fluorescence Filter Paddle | GM1100 |
| GloMax® Discover 96 Half-Position Aperture Assembly | GM1050 |
| GloMax® Discover 96-Position Aperture Assembly | GM3040 |
| GloMax® Discover 384-Position Aperture Assembly | GM3041 |
| GloMax® Discover Aperture Plate | GM3042 |
| GloMax® Dual Injectors with Pumps | GM3030 |
| Inner and Outer Injector Tubing, Natural | GM3014 |
| GloMax® Explorer Absorbance Module Upgrade | GM3520 |
| GloMax® Discover Sales Rental | E5301 |
| GloMax® Discover or Explorer Installation Qualification | SA1104 |
| GloMax® Discover or Explorer Operational Qualification | SA1105 |
| GloMax® Discover or Explorer Installation and Operational Qualification | SA1106 |
| GloMax® Discover Standard Service Agreement | SA4000 |
| GloMax® Discover or Explorer Preventive Maintenance | SA4030 |
| GloMax® Discover Premier Service Agreement | SA4002 |
| GloMax® Discover Premier Warranty Upgrade | SA4001 |
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
European Pat. No. 2972400 and Japanese Pat. No. 6324485.
Resources
Articles
- Measuring Blockade of Immune Checkpoint Targets with Promega Bioassays and GloMax® Discover
- Multiplexing with NanoDLRâ„¢ and CellTiter-Fluorâ„¢ Assays
- Measuring the ADCC Reporter Bioassay Complete Kit (WIL2-S) Signal on the GloMax® Discover System
- Lumit® Cytokine Assays: Interpolating Data with the GloMax® Discover
- Measuring GTPase, GEF and GAP Activities Using the GTPase-Glo™ Assay and the GloMax® Discover System
- Measuring Methyltransferase Activity Using MTase-Glo™ Assay and GloMax® Discover
- Using the NanoBiT® PPI System with GloMax® Discover to Detect Protein-Protein Interactions
- Detecting IL2 Pathway Activation with Promega Bioassays and GloMax® Discover
- PD-1+TIGIT Immune Checkpoint Detection with Promega Bioassays and GloMax® Discover
- Comparative characterization of different molecular formats of bispecific antibodies targeting EGFR and PD-L1
- Numb-associated kinases are required for SARS-CoV-2 infection and are cellular targets for antiviral strategies
- Evaluation of cfDNA as an early detection assay for dense tissue breast cancer
- An interaction between SLC35A1 and ST3Gal4 is differentially affected by CDG-causing mutations in the SLC35A1 gene
- Simultaneous inhibition of DNA-PK and PolÏ´ improves integration efficiency and precision of genome editing
- Chemokine CXCL4 interactions with extracellular matrix proteoglycans mediate widespread immune cell recruitment independent of chemokine receptors.
- ACLY as a modulator of liver cell functions and its role in Metabolic Dysfunction-Associated Steatohepatitis
- Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals
- Measure JmjC Demethylase and Fe(II)/α-KG-Dependent Hydroxylase Activity with Succinate-Glo™ Assay and GloMax® Discover
- Live-Cell NanoBRET Assay for AKT Inhibitor Binding
Software and Firmware
Other Resources
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