Single-Stranded DNA Binding Protein
Cooperatively Binds ssDNA with High Affinity
- Consists of four identical 18.9kDa subunits
- Does not bind well to double-stranded DNA
- Supplied at a concentration of 1–5µg/μl
Catalog Number:
Size
Catalog Number: M3011
E. coli Single-Stranded DNA Binding Protein (SSB) consists of four identical 18.9kDa subunits that cooperatively bind to single-stranded DNA with high affinity. This protein is involved in DNA replication and in recombination in vivo.
Applications
- Studying DNA replication and recombination.
- DNA sequencing using the primer walking strategy with contiguous oligonucleotides.
References
- Sancar, A. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4274–8.
- Chase, J.W. and Williams, K.R. (1986) Ann. Rev. Biochem. 55, 103–36.
- Krauss, G. et al. (1981) Biochemistry 20, 5346–52.
- Weiner, J.H. et al. (1975) J. Biol. Chem. 250, 1972–80.
Storage Buffer: 50mM Tris-HCl (pH 7.4), 1mM EDTA, 1mM DTT, 0.2M NaCl, 50% (v/v) glycerol.
Source: E. coli.
QC Tests: Protein concentration, DNase, RNase, SDS-PAGE/purity, endonuclease.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Single-Stranded DNA Binding Protein |
M301A | 1 × 100μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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