pGEM®-7Zf(+) Vector
Standard Cloning Vector and Template for In vitro Transcription and Circular ssDNA Production
- Blue/white screening for easily identifying recombinant clones
- In vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
- Multiple cloning region provides a convenient selection of restriction sites for cloning
Catalog Number:
Size
Catalog Number: P2251
The pGEM®-7Zf(+) Vector contains SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the α-peptide coding region of β-galactosidase. This arrangement of promoters means both sense and antisense RNA can be produced. Insertional inactivation of the α-peptide identified recombinant clones by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, ClaI, HindIII, BamHI, SacI, BstXI and NsiI.
Reference
- Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
pGEM®-7Zf(+) Vector |
P225A | 1 × 20μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for storage recommendations.
Resources
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