PKC nu Kinase Enzyme System
Easily Screen and Profile PKC nu Kinase Inhibitors
- Includes kinase, substrate and reaction buffer
- Use with ADP-Gloâ„¢ Assay for bioluminescent detection of kinase activity
Catalog Number:
Size
Additional options
Catalog Number: VA7534
Catalog Number: VA7535
Catalog Number: VA7536
Convenient, Scalable Kinase Profiling
The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PKC nu Kinase Enzyme System contains:
- PKC nu Kinase, 10μg (Human, full-length recombinant). MW: ~142kDa
- CREBtide substrate (1mg/ml), (KRREILSRRPSYR); derived from human CREB1 isoform A (amino acid 109–121)
- 5X Reaction Buffer, 0.1M DTT
Recombinant full-length human PKC nu was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. PKC nu, also known as PKD3, is a member of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKC nu is composed of 890 amino acid residues and the protein has 77.3% similarity to human PKCμ and 77. 4% similarity to mouse PKD.
The PKC nu Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Gloâ„¢ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Protocols
Complete Protocol
Specifications
Catalog Number:
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
ADP-Gloâ„¢ Kinase Assay |
V9101 | 1 × 1,000 assays |
PKC nu Kinase Enzyme System |
VA7534 | 1 × 10μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.U.S. Pat. No. 8,183,007 and other patents and patents pending.
U.S. Pat. No. 7,700,310 and other patents and patents pending.
Resources
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