Reporterassays + Transfektion
Luciferase Assays Produktgruppen
Genetic Reporter Vectors and Cell Lines
Reporter gene vectors including firefly, NanoLuc® and Renilla luciferases, GFP and dual-reporter coincidence vectors. Also find cell lines stably expressing firefly luciferase.
Reporter Assays
State-of-the-art bioluminescent assays. Reagents to detect firefly, Renilla, NanoLuc and Click-beetle luciferase activities.
Transfektionsreagenzien
Bewährte, gut zitierte Transfektionslösungen für eine Reihe von Zelltypen, darunter Primärzellen und iPS-Zellen.
Top Luciferase Assay Produkte für Ihr Labor
Nano-Glo® Dual-Luciferase Assay System
Ultra-sensitive detection of firefly and NanoLuc® luciferase activities in a single sample.
N1610, N1620, N1630, N1650, N1521, N1531, N1541, N1551
ONE-Glo™ EX Luciferase Assay System
Improved ONE-Glo™ Assay. Higher sensitivity, long-lived luminescence, more storage options.
E8110, E8120, E8130, E8150
FuGENE® HD Transfektionsreagenz
Verbesserte Leistung bei einer Vielzahl von Zelltypen, einschließlich schwer zu transfizierender Zelllinien.
E2311, E2312
Need a product modified?
Contact us to discuss custom and bulk formulations, custom packaging and automation options for bioluminescence-based assays and reagents.
What are Luciferase Assays?
Understanding how a promoter or response element may control gene expression is easier when studied with a reporter gene. Luciferase enzymes are ideally suited for reporter assays with simple light-based detection and high sensitivity that can quantify even small changes in expression. While reporter assays are often used to assess changes in promoter activity, they can also be used to investigate signal transduction, protein function, miRNA regulation and even real-time measurement of processes in living organisms.
The basis of a reporter assay is the vector, which carries the reporter gene, and the detection reagents that provide a substrate to convert the activity of the luciferase enzyme into a light signal. Dual-luciferase detection reagents quench the first reporter signal before reading the second to allow for two reporters to be measured sequentially from the same sample. The second reporter is often included as an internal control for more accurate data.
Once the reporter vector is selected and any elements cloned in (if needed), the vector is introduced into cultured cells, typically using transfection-based methods, expressed and activity detected with lytic (end-point) reagents or live-cell reagents for measuring changes in the same cells over time. Luciferase reporter assays can be multiplexed with other assays to assess apoptosis, metabolism and more. Measurement of luciferase assays is typically performed using a plate-based luminometer.
