Cell Health Assays
Wir bieten ein umfassendes Portofolio an Assays und Protokollen für die Analyse komplexer zellulärer und biochemischer Prozesse. Neben den bereits etablierten Assays zur Bestimmung der Zellviabilität, Zytotoxizität, Apoptose und oxidativem Stress, gibt es neue Assays für kinetische Messungen, die an Monolayern, 3D Zellkulturen, primären Zellen und Stammzellen angewendet werden können.
Cell Health Assays Produktgruppen
ADME-Tox Assays
Wählen Sie aus einer breiten Vielfalt an biolumineszenten Enzymsubstraten für Ihr ADME-Tox Screening aus.
Apoptose
Einfach anzuwendende Kits und Reagenzien als Apoptose-Nachweis, darunter Assays zum Multiplexen mit anderen Methoden, um mehrere Biomarker zu bestimmen.
Autophagie
Plattenbasierte Methode zur quantitativen Bestimmung des Ablaufs der Autophagie.
Zellviabilität & Zytotoxizität
Real-time und Endpoint, Add-Mix-Measure Assays.
Inflammation
Eine einfache Methode zur selektiven Messung der Caspase 1-Aktivität, einem essenziellen Bestandteils des Inflammasoms.
Oxidativer Stress
Assays zur Bestimmung reaktiver Sauerstoffspezies sowie zur Messung des Verhältnisses von reduziertem zu oxidiertem Glutathion.
Top Cell Health Assays Produkte für Ihr Labor
CellTiter-Glo® 2.0 Cell Viability Assay
Updated CellTiter-Glo® Cell Viability Assay with improved reagent stability. Quantifies cell proliferation based on ATP detection.
G9241, G9242, G9243, MG1010
Caspase-Glo® 3/7 Assay System
An easy-to-use, plate-based luminescent assay for detecting caspase-3/7 activity.
G8090, G8091, G8093, G8092, MG1010
LDH-Glo™ Cytotoxicity Assay
Bioluminescence-based LDH assay for sensitive detection of cytotoxicity in samples low in cell number, including 3D microtissues.
J2380, J2381, MG1010
An Introduction to Cell Health Assays
Cell health assays are used to detect and monitor various cellular events and build a picture of how and why cells live or die. They are used for many different applications including assessing toxicity of drug candidates and other compounds, analyzing cellular changes in cancer and other diseases, and studying basic cell biology.
Important factors to consider when choosing a cell health assay include the parameter you want to measure, the sensitivity needed, the model system you are using, the desired endpoint, number of samples, and the equipment or detection instrumentation required.
There are cell health assays that detect the number of living cells (viability assays), the number of dead cells (cytotoxicity assays) and for assessing the mechanism of cell death (apoptosis assays). Cell viability and cytotoxicity assays easily confirm the difference between no treatment and treatment of experimental wells. Additional assay endpoints such as caspase activation or phosphatidyl serine exposure, or multiplexing of multiple cell health endpoints, help elucidate more information on the impact of the treatment on the cells. In addition to proliferation or cytotoxicity, apoptosis, necrosis and cytostatic responses can all be evaluated using cell health assays.
Cells in culture are only a model system and are different than cells in their normal in vivo environment. 3D cell cultures more closely mimic in vivo conditions but can present challenges for experimental design. Assay reagents may have difficulty reaching the center of large 3D microtissues, and lytic assays may not be able to disrupt all cells within the 3D system. If using 3D cultures, products and assay protocols optimized for 3D systems will be a better choice.
Assay formats for cell health assays include endpoint and real time. Endpoint assays provide a snapshot in time of what is occurring at a cellular level and are suited to applications where treatment response time is understood. Real-time assays provide repeated measurements from the same sample over time, are helpful in understanding the time course of a response and conserve cells and samples.
