Citations Search

Notes: These authors used the Maxwell® 16 Tissue DNA Purification Kit to extract DNA from 200µl samples of raw meat homogenate from beef, pork, poultry and lamb samples. They also used the Wizard® Genomic DNA Isolation System to extract DNA from cooked meat samples. The extracted DNA was used in real-time, quantitative PCR assays to identifiy species-specific DNA spiked at 1% in mixed DNA samples. (4353)

Notes: These authors used the Maxwell^® 16 Blood DNA Purification Kit to isolate genomic DNA from leukocytes. (4210)

Notes: These authors used the Maxwell^® 16 System to extract DNA from multiple sample collection devices containing a model microbial community (MMC) comprised of 11 distinct species of bacterial, archaeal and fungal lineages associated with spacecraft or clean-room surfaces. The authors compared cotton swabs, polyester wipes and biological sampling kits to assess the success of recovering DNA of rRNA genes for species-specific PCR analysis. (4124)

Notes: These authors compared the performance of automated DNA purification using the Maxwell® 16 Instrument with manual methods. DNA yield and quality from milk, blood (buffy coat), and semen samples from cattle were evaluated. The Maxwell® 16 System performed best, consistently generating high quantity and quality DNA across the sample range tested. (4213)

Notes: These authors investigated the relationship between calorie restriction and INDY expression on lifespan in Drosophila melanogaster. They showed that calorie restriction downregulates INDY expression in normal flies. INDY mutants on a normal diet had increased lifespan that was not extended further by calorie restriction. The INDY long-lived flies also shared several phenotypic characteristics with normal flies on a calorie-restricted diet. The authors then demonstrated that the phenotypic effects of the INDY mutation were not caused by reduced ability to intake food. The results show that INDY and calorie restriction interact to extend lifespan, and that decreased INDY expression induces a calorie-restriction-like status. During the study, the Maxwell 16 Tissue DNA Purification System was used to isolate DNA from Drosophila. The DNA was used in PCR analyses to confirm the absence of Wolbachia DNA in the experimental lines. (4001)

Notes: These authors used the Maxwell^® 16 System to isolate genomic DNA from whole blood and normal colonic tissue samples. The DNA was used in genotype analysis, testing for wildtype and mutant KRAS genes, and for various EGFR-related polymorphisms. The results were used in a research study testing the relationship between various genotypes and response to different treatment regimens. (3961)

Notes: These authors used the Maxwell^® 16 System to isolate DNA from frozen whole blood samples infected with Plasmodium falciparum. The isolated DNA was used in a qPCR-based SNP genotyping assay that sought to uniquely identify the parasites based on their SNP marker profile. (3962)

Notes: These authors used the Maxwell^® 16 Total RNA Purification Kit and the Maxwell^® 16 Instrument to purify total RNA from exponentially grown Legionella pneumophila for use in primer extension assays. (3798)

Notes: In this study, the Maxwell^® 16 Instrument was used to purify genomic DNA from blood samples. The extracted DNA was amplified by PCR for subsequent genotype analysis. (3902)

Notes: These authors evaluated 225 tumor samples from various ovarian and peritoneal tumor types for expression of the epidermal growth factor receptor type III variant EGFRvIII. EGFvIII has not been observed in normal tissues and so was evaluated as a potential target for tumor-specific therapies. However, none of the 225 samples evaluated were positive for this marker, suggesting that the EGFRvIII mutation is rare in ovarian tissue and is not a good therapeutic target for this disease. The authors used the Maxwell 16 Total RNA Purification Kit and the Maxwell 16 Instrument to purify RNA from tissue samples that had been fresh-frozen and fixed in the stabilization reagent RNAlater (Qiagen). Complete details of the RNA purification procedure are provided in the paper. (3878)

Notes: In this study, the Maxwell^® 16 Instrument and Maxwell^® 16 DNA Purification Kit were used to isolate DNA from cell lines derived from head and neck squamous cell carcinomas. (3891)

Notes: This paper describes analysis of DNA samples at a mock crime scene using automated DNA purification followed by STR analysis on a portable microchip system. The mock crime scene was created using "victim" and "suspect" blood stains prepared by spotting 3µl of liquid blood onto paper towels and a cloth shirt. The samples were allowed to dry overnight before placement at the crime scene. Samples were processed at the scene using the Maxwell^® 16 Instrument and DNA IQ™ Casework Sample Kit for DNA extraction, and a microchip analyzer to perform amplification and STR analysis. The 9-plex autosomal STR typing system used in the microchip system included primer sequences from the PowerPlex^® 16 System (D3S1358, THO1, D21S11, D5S818, D13S317, D7S820, vWA and D8S1179) and amelogenin for sex identification. DNA purification from suspect samples was completed in 2 hours, and subsequent STR analysis and generation of a "suspect" DNA profile took a further 3 hours. The entire process from sample collection to generation of a CODIS "hit" took 6 hours. (3922)

Notes: These authors describe a simple, small-scale screening method for recombinant polyhistidine-tagged proteins. They used the Maxwell^® 16 Polyhistidine Protein Purification Kit and Maxwell^® 16 Instrument to purify the proteins, and characterized the purified proteins by NMR and X-Ray analysis. They first used the Flexi^® Vector System to clone the genes of interest into expression vectors containing either an N-terminal TEV protease cleavable His8-Maltose Binding Protein (His8-MBP) tag, or an in vivo cleaved His8-MBP tag. For small-scale screening, E. coli expressing fusion proteins were grown for 24 hours in auto-induction medium in 96-well growth blocks, harvested by centrifugation and resuspended to an OD₆₀₀ of 20 in 1ml of 50mM HEPES (pH 7.5) containing a protease inhibitor cocktail. Aliquots of these cells were then added directly to the Maxwell^® kit cartridge, and automated purification performed. The purified proteins were analyzed by SDS-PAGE or using a Caliper LC90 electrophoresis system. For purification of proteins for use in NMR or crystallography studies, 50ml overnight cultures were harvested and resuspended in 10 ml of 50mM HEPES (pH 7.5) containing 10 units of benzonase, 1mg/ml lysozyme, and protease inhibitor cocktail. The cell suspension was sonicated, and aliquots were then added to the Maxwell^® 16 cartridge for purification. The authors purified 14 different proteins from humans, frogs, and zebrafish using this method. Detailed reports of yields obtained and subsequent analyses are provided in the paper. (3799)