Technical Reference

technical reference

• Abbreviations (PDF 255 KB)

  Definitions for commonly used molecular biology abbreviations.

• Absorbance, Excitation and Emission Spectra for Bioluminescent Assays (PDF 206 KB)

  Physical Characteristics of Bioluminescent Assays from Promega

• Absorbance, Excitation and Emission Spectra for Fluorescent and Spectrophotometric Assays (PDF 218 KB)

  Absorbance, Excitation and Emission Spectra for Fluorescent and Spectrophotometric Assays.

• Agarose and Polyacrylamide Gels: Dye Migration and Sample Resolution (PDF 206 KB)

  Dye migration and sample resolution in gels of varying percentage.

• Amino Acid Abbreviations and Molecular Weights (PDF 250 KB)

  Amino acid abbreviations, structures and codon usage tables.

• Antibiotic Mode of Action and Resistance Mechanisms (PDF 203 KB)

  Mode of action, working concentrations, and resistance mechanisms for commonly used antibiotics.

• Antibody Cross-Reactivity Tables (PDF 226 KB)

  Tables that list Promega antibodies, its usage in various applicatons and species cross-reactivity.

• Genetic Markers of E. coli (PDF 268 KB)

  Description of genetic markers in commonly used E. coli strains.

• Genotypes of Frequently Used Bacterial Strains (PDF 242 KB)

  Genotypes of Frequently Used Bacterial Strains

• Isoschizomers (PDF 274 KB)

  Isoschizomers

• Label Symbols Key (PDF 64 KB)

  An explanation of the symbols used on Promega product labels.

• Lysis Buffer Compatibility with Reporter and Protein Assays (PDF 232 KB)

  Compartibility of Promega lysis buffers with reporter assays, protein assays and Western blotting systems.

• Nucleotides and Nucleic Acids (PDF 258 KB)

  Nucleotide physical constants, ambiguity code and structure. Copy number and molecular weight of common nucleic acids

• Oligonucleotide Molecular Weight and Molarity (PDF 223 KB)

  Reference information on Promega primers and oligos.

• DNA and Protein Calculations (PDF 203 KB)

  Common conversions and formulas for DNA and protein conversions.

• Primer Design Considerations for Adding a T7 Promoter to a PCR Product (PDF 210 KB)

  Primer design considerations for incorporating a T7 promoter into a PCR product for in vitro translation.

• Properties of Thermostable DNA Polymerases (PDF 203 KB)

  Molecular weight, A-tailing ability, proofreading activity and other characteristics of common thermostable polymerases.

• Radioisotope Properties (PDF 200 KB)

  Physical properties of beta, gamma and X-ray emitting radionuclides.

• Recipes for Common Laboratory Solutions (PDF 247 KB)

  Recipes for buffers and other common laboratory solutions.

• Restriction Enzyme Buffer Composition (PDF 205 KB)

  Composition of Promega Restriction Enzyme Buffers A-L

• Restriction Enzyme Activity in Promega Buffers (PDF 929 KB)

  Buffer compatibility chart for all Promega restriction enzymes.

• Restriction Enzymes: Cutting Near the End of PCR Fragments (PDF 211 KB)

  Considerations for design of primers containing restriction sites.

• Sequencing Primers (PDF 237 KB)

  Tm and applicable vectors for promega sequencing primers.

• Site-Specific Methylation of Promega Restriction Enzymes (PDF 229 KB)

  Methylation sensitivities of restriction enzymes.

• Strain Restriction Phenotypes (PDF 213 KB)

  E. coli strain restriction phenotypes.

• Temperature Dependence of pH for Common Buffers (PDF 201 KB)

  Temperature Dependence of pH for Common Buffers