Promega Corporation

KRX Autoinduction Protocol A Convenient Method for Protein Expression

Share

  • Share
  • Print
  • Email
  • Download PDF

Abstract

Recombinant protein expression in bacteria is a simple, yet time-consuming process. Typically cells are grown overnight to stationary phase, diluted and grown to an optimal density, then induced. To simplify this process, we took advantage of the tight regulation of protein expression by glucose and rhamnose in the KRX strain of E. coli. We developed an autoinduction protocol that eliminates the need for culture density monitoring and a separate induction step. We describe early and late autoinduction protocols in LB medium and demonstrate autoinduced expression of three different proteins.

Promega Notes 98, 16–18.

Trista Schagat, Rachel Ohana, Paul Otto, Jim Hartnett and Michael Slater

Promega Corporation
Publication Date: 2008

Download Article (317 KB)

Prefer a different language?

Ihr ausgwähltes Land istAustria. Ihre ausgewählte Sprache istDeutsch. Bitte wählen Sie die Sprache aus:

Dies ist richtig, bitte weiter gehen zu »

Ich brauche weitere Hilfe

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.