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Recent Articles

Measuring Intracellular Protein Lifetime Dynamics Using NanoLuc® Luciferase

Single-Neuron Gene Expression Analysis Using the Maxwell® 16 LEV System in the Neural Systems and Behavior Course

Optimized Reagents for Probe-Based qPCR using the GoTaq® Probe qPCR and RT-qPCR Systems

Determine Viral-Induced Cytopathic Effect Using a Luminescent Assay

RNA Purification from BioMatrica RNAgard® Blood Tubes Using the Maxwell® 16 LEV simplyRNA Blood Kit

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LabFact #35

When expressing your protein in a cell-free system, make sure the combination of promoter and DNA conformation is appropriate. For example, the T7 RNA polymerase promoter may express better in a linearized template while the SP6 promoter transcribes better in a circular DNA.

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by Ed Himelblau

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