GoScript™ Reverse Transcription System Technical Manual

Literature # TM316

The GoScript™ Reverse Transcription System is a convenient kit that includes a reverse transcriptase and an optimized set of reagents for efficient synthesis of first-strand cDNA optimized in preparation for PCR amplification. The components of the GoScript™ Reverse Transcription System can be used to reverse transcribe RNA templates starting with either total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction buffer and reverse transcriptase provided in the GoScript™ Reverse Transcription System enable robust, full-length cDNA synthesis for reproducible analysis of rare or long messages. These conditions were developed for cDNA synthesis or for easy transition to gene-specific target amplification. Up to 5μl of the reverse transcription reaction can be directly amplified using Taq DNA polymerase in a 25µl PCR. GoScript™ Reverse Transcriptase is qualified for use in two-step RT-qPCR, using GoTaq® qPCR and Plexor® qPCR Systems. In two-step RT-qPCR using GoTaq® qPCR Master Mix, samples of the GoScript™ Reverse Transcription System can be added directly, up to 20% v/v.