SAM²® Biotin Capture Membrane Technical Bulletin

Literature # TB547

The SAM²® Biotin Capture Membrane binds biotinylated molecules based on their affinity for streptavidin. The proprietary process by which the SAM²® Membrane is produced results in a high density of streptavidin on the filter, providing rapid, quantitative substrate binding in the nmol/cm² range. In addition, the membrane is optimized for low nonspecific binding. The membrane is available either as a large, prenumbered, partially cut sheet (approximately 10.5 × 15.0cm; Cat.# V2861) or as a smaller, uncut sheet (approximately 7.6 × 10.9cm; Cat.# V7861). The partially cut membrane (Cat.# V2861) allows easy separation into 96 individual squares and is designed for small-scale experiments where high binding capacity is required. The uncut sheet (Cat.# V7861) can be analyzed as a whole membrane or may be cut into the size desired. The uncut membrane allows sample application using a multichannel pipettor. Both membranes may be analyzed using phosphorimaging, autoradiography or scintillation counting to quantitate results. The membranes also have been used successfully with chemiluminescence detection techniques. The use of fluorescence to detect captured molecules is not recommended at this time. The SAM²® Membrane, used as recommended in this technical bulletin, provides a number of advantages over other commercially available streptavidin products.