CytoTox-ONE™ Homogeneous Membrane Integrity Assay Technical Bulletin

Literature # TB306

The CytoTox-ONE™ Homogeneous Membrane Integrity Assay is a homogeneous, fluorometric method for estimating the number of nonviable cells present in multiwell plates. Cell viability is most often defined based on cell membrane integrity and is most commonly measured by observing the exclusion of Trypan blue or other vital dyes. Measuring leakage of components from the cytoplasm into the surrounding culture medium has been widely accepted as a valid method to estimate the number of nonviable cells. The CytoTox-ONE™ Assay is a rapid, fluorescent measure of the release of lactate dehydrogenase (LDH) from cells with a damaged membrane. LDH released into the culture medium is measured with a 10-minute coupled enzymatic assay that results in the conversion of resazurin into resorufin. The CytoTox-ONE™ Reagent mix does not damage healthy cells; therefore, researchers can measure released LDH directly in assay wells containing a mixed population of viable and damaged cells.