SignaTECT® DNA-Dependent Protein Kinase Assay System Technical Bulletin

Literature # TB250

The SignaTECT® DNA-Dependent Protein Kinase Assay System provides an improved method to quantitate DNA-dependent protein kinase (DNA-PK) activity in both purified enzyme preparations and cell nuclear extracts. The SignaTECT® System uses the unique SAM²® Biotin Capture Membrane, produced by a proprietary process that results in a high density of streptavidin on the membrane matrix.

The SignaTECT® DNA-PK Assay System overcomes the problem of nonspecific substrate binding by using a biotinylated DNA-PK p53-derived peptide substrate in conjunction with the SAM²® Biotin Capture Membrane. The high binding capacity of the SAM²® Biotin Capture Membrane for the DNA-PK biotinylated peptide substrate and low backgrounds observed with this system maximize the signal-to-noise ratio.