pGEM®-7Zf(+) Vector Technical Bulletin

Literature # TB048

The pGEM®-7Zf(+) Vector is a derivative of the pGEM®-3Zf(+) Vector and contains the origin of replication of the filamentous phage f1. The plasmid serves as a standard cloning vector, as a template for in vitro transcription and as a template for producing circular ssDNA. The plasmid contains SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the alpha-peptide coding region of beta-galactosidase. Insertional inactivation of the alpha-peptide allows recombinant clones to be identified directly by color screening on indicator plates. The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, Csp45I, ClaI, HindIII, BamHI, SacI, BstXI and NsiI. This arrangement is designed specifically for generating unidirectional deletions with the Erase-a-Base® System. The polylinker contains restriction enzyme sites that produce 5´ overhangs or blunt ends (sensitive to exonuclease III) flanked on both sides by blocks of restriction sites that generate 3´ overhangs (resistant to exonuclease III).