The power of the polymerase chain reaction (PCR) is greatly enhanced by techniques that provide accurate, sensitive and reproducible quantitative data. These real-time or quantitative PCR (qPCR) techniques determine the amount of input template based on the accumulation of PCR product during the exponential phase of an amplification reaction. In this article we introduce the Plexor™ Systems, which use a novel technique for real-time PCR that that requires only two primers for sensitive and specific quantitation.
Promega Notes 92, 10–13.
Susan Frackman, Benjamin Krenke, Katharine Hoffmann, Cindy Sprecher, Kyle Hooper, Doug Storts