The GeneClip™ U1 Hairpin Cloning Systems enable cloning and expression of short hairpin RNAs (shRNAs) for RNA interference (RNAi) experiments. The GeneClip™ Systems contain linearized vectors that facilitate cloning of complementary oligonucleotides downstream of a U1 promoter. Upon transfection with pGeneClip™ Vectors containing these cloned sequences, shRNA is produced in the cell, leading to reduction of target gene expression through RNAi. In this article we illustrate use of the GeneClip™ Systems to suppress Renilla luciferase expression in a range of cell types and to suppress p53 expression in both a transient and stable manner.
Promega Notes 89, 21–24.
Thomas Yeager, Cheryl Bailey, Jolanta Vidugiriene, Brian Almond and Doug Storts