We have developed an optimized protocol for generating short interfering RNAs (siRNAs) or hairpin siRNAs in vitro using T7 RNA Polymerase and annealed DNA oligonucleotide templates. Two RNA interference studies in different mammalian model systems demonstrate the functionality of the synthesized siRNAs. The efficient and inexpensive synthesis of siRNAs in vitro allows for quick screening of multiple target sites to identify the siRNA that produces the most potent silencing effect.
Promega Notes 85, 15–18.
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