We describe modifications of two current genomic DNA purification systems to address researchers' needs for automated plant genomic DNA purification. We have developed methods in a 96-well format to obtain greater than 1µg of genomic DNA from leaf or seed as well as methods to obtain a specific yield of genomic DNA from plant tissue. These methods improve productivity by providing sufficient DNA for multiple assays from a single purification or by obtaining a fixed yield of DNA, eliminating the need to quantitate and normalize before an assay.
Promega Notes 83, 25–29.
Susan Koller, Rex Bitner and Hemanth Shenoi