Promega offers the GeneClip™ and siCHECK™ Vectors for use in RNA interference studies. The GeneClip™ Vectors are used to express shRNAs in vivo. The siCHECK™ Vectors provide luciferase-reporter based indicators of RNAi activity.
GeneClip™ U1 Hairpin Cloning
Linearized plasmids designed for fast and easy cloning of human target sequences to express short hairpin RNAs (shRNAs) in human cells. Vectors are supplied predigested to eliminate time-consuming preparation.
Rapidly screen RNAi by quantifying the Renilla luciferase reporter gene activity. Avoid labor-intensive, time-consuming assays and waiting for phenotypic changes.
This chapter of the Protocols and Applications Guide discusses the design and optimization of siRNA sequences, interference optimization, and delivery strategies for siRNA.
In this article, we present data demonstrating the utility of the psiCHECK™-2 Vector as a biosensor for microRNA activity in cells.
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