Prokaryotic Cell-Free Protein Expression

S30 T7 High-Yield System

Express up to 500µg/ml protein directly from plasmid DNA in only 1 hour with this E. coli-based system.

• Protocol
• Product Details + Ordering

T7 S30 System for Circular Templates

To express protein, add plasmid DNA containing a T7 promoter and a ribosomal binding site to the S30 extract. No purification required after expression. Use directly for proteomics related experiments.

• Protocol
• Product Details + Ordering

S30 Extract System for Linear Templates

Express protein quicky and easily from PCR, DNA fragments, ligated overlapping oligonucleotides, in vitro generated RNA, or prokaryotic RNA.

• Protocol
• Product Details + Ordering

In this article we discuss factors that improve protein yield with the S30 T7 High-Yield Protein Expression System.