Promega Corporation

Multimode Readers Comparison Guide

  GloMax-Multi+ Microplate with Instinct Software GloMax-Multi Microplate GloMax-Multi Jr Single Tube
  GloMax®-Multi+ Microplate
with Instinct® Software
GloMax®-Multi
Microplate
GloMax®-Multi Jr
Single Tube
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Catalog No. E8032 with Shaking
E9032 with Heating and Shaking
E7031 E6070 Base Instrument
E6080 Instrument with Luminescence Installed
Product Type 6-384-well Microplate Multimode Reader 96-well Microplate Multimode Reader Single Tube Multimode Reader
Description Versatile Microplate Reader with Shaking, Instinct® Software, and Optional Heating.
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State-of-the-art optics, instrument design and data handling. Superior performance, affordable with flexible purchasing options.
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Multifunctional single tube instrument for utmost flexibility.
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Available Detection Modes Luminescence:
E8041 Luminescence
Fluorescence:
E8051 Fluorescence
Absorbance:
E8061 Visible
E9061 UV Visible
Luminescence:
E7041 Luminescence
Fluorescence:
E7051 Fluorescence
Absorbance:
E7061 Visible
Luminescence:
E6098 Luminescence (or installed in instrument E6080)
Fluorescence:
E6071 Blue
E6072 UV
E6073 Green
E6074 Red
E6075 GFPUV
Absorbance:
E6076 Absorbance Module. Add 1 or more filter paddle(s):
E6077 560nm Filter
E6078 600nm Filter
E6079 750nm Filter
Read Type Glow, Flash, Kinetic, Repeat Glow, Flash, Kinetic, Repeat Glow, Kinetic, Repeat
Sample Format 6, 12, 24, 48, 96, 384-well plates 96-well plates 10 x 10 mm cuvette, 100 - 200 µl minicell vial (optional), 0.5 ml PCR tube (optional, fluorescence), 1.5 ml microcentrifuge tube (Luminescence)
Shaking Four speeds (150, 300, 500 rpm, Variable) linear and orbital modes Not Available Not Available
Temperature Control Optional: 2° C above ambient temperature to 45° C +/- 0.75° C Not Available Not Available
Injectors Optional:
E7071 Single Injector
E7081 Dual Injectors

Selectable between 25 - 200 µl in 5 µl increments for 6 to 96-well plate formats.
Optional:
E7071 Single Injector
E7081 Dual Injectors

Selectable between 25 - 200 µl in 5 µl increments.
Not Available
Luminescence      
Detector Head-on photomultiplier tube (PMT) for photon-counting Head-on photomultiplier tube (PMT) for photon-counting Photomultiplier tube (PMT)
Detection Limit 3 x 10-21 moles of Luciferase or 1 x 10-18 moles of ATP 3 x 10-21 moles of Luciferase or 1 x 10-18 moles of ATP 1 x 10-16 moles ATP or 1 x 10-20 moles of Luciferase
Linear Dynamic Range > 8 logs > 8 logs 5 logs
Crosstalk 5 x 10-5 5 x 10-5 Not Applicable
Sample Adapter Not Applicable Not Applicable Optional. Holds 1.5 ml microcentrifuge tubes
Spectral Range 350 - 650 nm 350 - 650 nm 350 - 650 nm
Peak Wavelength 420 nm 420 nm 420 nm
Fluorescence      
Light Source Wavelength-matched LED Wavelength-matched LED Wavelength-matched LED
Detector PIN-photodiode PIN-photodiode Photodiode
Read Position Top reading Top reading 90 degree
Wavelength Selection Snap-in Fluorescence Optical Kits Snap-in Fluorescence Optical Kits Snap-in Fluorescence Modules (user installable)
Wavelengths UV (Ex 365 nm: Em 410 - 460 nm)

Blue (Ex 490 nm: Em 510 - 570 nm)

Green (Ex 525 nm: Em 580 - 640 nm)

Red (Ex 625 nm: Em 660 - 720 nm)

AFC (Ex 405 nm: EM 495 - 505 nm)
UV (Ex 365 nm: Em 410 - 460 nm)

Blue (Ex 490 nm: Em 510 - 570 nm)

Green (Ex 525 nm: Em 580 - 640 nm)

Red (Ex 625 nm: Em 660 - 720 nm)

AFC (Ex 405 nm: EM 495 - 505 nm)
UV (Ex 365 nm: Em 410 - 450 nm)

Blue (Ex 460 nm: Em 515 - 570 nm)

Green (Ex 525 nm: Em 580 - 640 nm)

Red (Ex 625 nm: Em 660 - 725 nm)

UV-GFP (Ex: 365 nm, Em: 515 - 570 nm)
Detection Limit 0.5 fmol/200 µl or 1 ppt of fluorescein in 96-well plate, 30 pg/well dsDNA using DNA Quantation Dye 0.5 fmol/200 µl or 1 ppt of fluorescein in 96-well plate, 30 pg/well dsDNA using DNA Quantation Dye 6 ppt fluorescein, 450 pg/ml DNA using DNA Quantation Dye
Linear Dynamic Range 6 logs, assay dependent 6 logs, assay dependent 6 logs, assay dependent
Read Out Relative Fluorescence Units, Direct Concentration Relative Fluorescence Units Fluorescence Standard Units, Direct concentration
Calibration     1 to 5 point calibration
Discrete Sample Averaging Sample readings are averaged over 0.5 second to improve accuracy Sample readings are averaged over 0.5 second to improve accuracy Sample readings are averaged over 5 seconds to improve accuracy
Visible Absorbance      
Light Source LED LED LED
Detector Large-area photodiode Large-area photodiode Photodiode
Spectral Range 450 - 750 nm 450 - 750 nm 400 - 800 nm
Wavelengths for Installed Filters 450, 560, 600, 750 nm, 2 slots for customization 450, 560, 600, 750 nm, 2 slots for customization 560 nm, 600 nm, 750 nm
Photometric Measuring Range 0 - 5.0 OD 0 - 5.0 OD 0 - 4.0 OD
Linear Dynamic Range 0 - 4.0 OD, assay dependent 0 - 4.0 OD, assay dependent 0 - 2.5 OD, assay dependent
OD Accuracy 0.01 OD ± 3% at ≤ 2.5 OD 0.01 OD ± 3% at ≤ 2.5 OD ±0.7%
OD Precision 0.01 OD ± 1% 0.01 OD ± 1% ±0.5% at 1.0 OD
Stray Light 0.002% at 560 nm in clear bottom, black wall plate 0.002% at 560 nm in clear bottom, black wall plate Not Applicable
UV-Visible Absorbance      
Light Source Xenon Lamp Not Applicable Not Applicable
Detector Photodiode Not Applicable Not Applicable
Spectral Range 200 - 1100 nm Not Applicable Not Applicable
Wavelengths for Installed Filters 260, 280, 450, 560, 600, 750 nm (2 slots customizable) Not Applicable Not Applicable
Photometric Measuring Range 0 - 4.0 OD Not Applicable Not Applicable
Linear Dynamic Range 0 - 3.5 OD, assay dependent Not Applicable Not Applicable
OD Accuracy 0.05 OD ± 3% at ≤ 2.5 OD Not Applicable Not Applicable
OD Precision 0.002 ± 3% Not Applicable Not Applicable
Stray Light 0.001% at 560 nm in clear bottom, black wall plate Not Applicable Not Applicable
Instrument      
User Interface Built-in PC, touch screen navigation and operation Built-in PC, touch screen navigation and operation Touch screen navigation and operation
Data Output PC compatible ASCII text file format exported to USB flash drive, or connect to PC (not included) through USB or Serial Port PC or Mac compatible csv file format exported to USB flash drive, or connect to PC (not included) through optional PC Connect Kit Data displayed on screen or connect to PC (not included) via serial cable to download. Optional thermal printer available
External PC Requirements Windows XP or higher Windows XP or higher Windows XP SP2 or later
Computer Interface USB type B or RS-232 port USB type B or RS-232 port 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud
Power 100 - 240 VAC, 50 - 60 Hz 100 - 240 VAC, 50 - 60 Hz Instrument: 12V DC, 0.7A Max, AC Adapter: 100-240V AC, 50/60Hz, 0.3A
Auto-Shutoff Touch screen hibernates after 15 minutes of inactivity Touch screen hibernates after 15 minutes of inactivity Touch screen hibernates after 15 minutes of inactivity
Information      
Application Notes View Application Notes View Application Notes View Application Notes
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