I-PpoI (Intron-Encoded Endonuclease)

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I-PpoI Protocol MSDS Components Add to Helix Close Window I-PpoI  Components Bovine Serum Albumin, Acetylated R396A1 x 1,000μl I-PpoI R703A1 x 10,000u I-PpoI Positive Control Plasmid R707A1 x 10μg I-PpoI 10X Buffer R994A1 x 1ml Close Window This product can be added to a Helix Freezer This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft. Click here to learn more about the Helix Collection » Already a Helix Customer? Login to request to add this product to your Helix »	10,000u	100–200u/μl	R7031	€ 103,00		Add to cart

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Frequency of Cutting

No sites are present in any of the commonly used plasmids or vectors. One site is present in chromosome 12 of S. cerevisiae DNA.

Incubation Conditions

I-PpoI Buffer. 37°C.

Percent Activity in 4-CORE^® Buffer

A	B	C	D	I-PpoI
10–25%	25–50%	25–50%	25–50%	100%

Note: A precipitate may be observed after thawing. If detected, heat to 37°C for 3–5 minutes and mix to dissolve. The buffer may appear cloudy after heating; this will not affect the performance of the product.

Source

I-PpoI is an intron-encoded endonuclease from Physarum polycephalum. The enzyme is produced from a recombinant E. coli strain.

Storage Buffer

50mM Tris-HCl (pH 7.5), 0.01% NP-40, 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

R7031 For Research Use Only. Not for Use in Diagnostic Procedures.