Bacterial Strain LE392 is used for both genomic and cDNA cloning. LE392 lacks the E. coli K restriction system but is rec+. It is a permissive host, allowing both recombinant and parental phage to grow. Unlike Y1090, LE392 does not allow color selection of recombinants or IPTG induction of expression. Also, since LE392 contains lon protease activity, fusion proteins are often less stable in this host than in Y1090. When using this strain, phage titers tend to be higher than with Y1090 host cells. Therefore, we recommend (if color selection is not required) that LE392 be the primary strain for amplification of re...
Expand to Read More »
Bacterial Strain LE392 is used for both genomic and cDNA cloning. LE392 lacks the E. coli K restriction system but is rec+. It is a permissive host, allowing both recombinant and parental phage to grow. Unlike Y1090, LE392 does not allow color selection of recombinants or IPTG induction of expression. Also, since LE392 contains lon protease activity, fusion proteins are often less stable in this host than in Y1090. When using this strain, phage titers tend to be higher than with Y1090 host cells. Therefore, we recommend (if color selection is not required) that LE392 be the primary strain for amplification of recombinant phage and for screening a cDNA library with nucleic acid probes.
Genotype: hsdR514(rk–, mk+), glnV(supE44), tryT (supF58), lacY1 or Δ(lacIZY)6, galK2, galT22, metB1, trpR55.
« Collapse Content
Bacterial Strain LE392, Glycerol Stock
Bacterial Strain LE392
Store at –70°C.
For product intended use please see Patents & Disclaimers tab.
K9981 For Research Use Only. Not for Use in Diagnostic Procedures.
Your country is set to Austria. Your language is set to Deutsch. Please select the language that will best suit your needs:
This is correct, continue to site »
I need additional help